Cell-dependent susceptibility to Porcine circovirus ORF3-induced apoptosis

21-10-2010 | | |

Porcine circovirus associated disease (PCVAD) has caused considerable economic impact worldwide since its first description on a Canadian pig farm in the 1990s. PCVAD, a wasting and immunosuppressive disease in post-weaned pigs, is caused by Porcine circovirus type 2 (PCV2).

Despite the availability of effective vaccines, little is known about the molecular pathogenesis of the disease. Belonging to the Circoviridae family, PCV2 and PCV1 are closely-related viruses with a 1.7kb, ambisensed and circular ssDNA genome 1. While PCV2 is pathogenic, PCV1 is not.
Investigating the differences in the genomic composition of these viruses will provide clues to the molecular pathogenesis of PCVAD. ORF3 is a good candidate as a determinant of PCV pathogenicity because PCV1 and PCV2 ORF3 share only 60.6% amino acid (aa) sequence identity in the translated region. In addition, a consistent single nucleotide (nt) substitution in the PCV2ORF3 coding region resulted in a stop codon, leading to PCV2ORF3 that is half the length
Mark Chaiyakul1 Karolynn Hsu1 Avril Hatherell1 Rkia Dardari1 Frank Marshall1, 2 Markus Czub1
1. University of Calgary, Calgary, AB, Canada; 2. Swine Health Services, Calgary, AB, Canada
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